Evaluation of the Spectrophotometric Assay of Guanase with 2,2'-Azino-di(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as Chromogen
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چکیده
منابع مشابه
Evaluation of the spectrophotometric assay of guanase with 2,2'-azino-di(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as chromogen.
A simple spectrophotometric assay for serum guanase based on the oxidation of 2,2'-azino-di(3-ethylbenzthiazoline-6-sulphate) (ABTS) using xanthine oxidase, uricase and peroxidase is described and statistically examined through its application to normal and pathological sera. The method is very sensitive, precise (CV below 8.13%) and linear up to 152.5 U/l. Comparison with the methods of Hue & ...
متن کاملColorimetry of hemoglobin in plasma with 2,2'-azino-di(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS).
Hemoglobin in plasma can be determined by the color-developing action of 2,2'-azino-di(3-ethylbenzthiazoline-6-sulfonic acid), which is oxidized to a colored form by a peroxidase-like effect of hemoglobin in the presence of hydrogen peroxide. Sensitivity, precision, and accuracy are discussed. The calibration curve is linear for hemoglobin concentrations up to 1 g/L; the minimum detectable conc...
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15 صفحه اولThe steady-state kinetics of peroxidase with 2,2'-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid) as chromogen.
The chemical nature of the important new chromogen ABTS [2,2'-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid)] is described together with an account of the redox and spectroscopic properties of the system ABTS--H2O2--peroxidase. Keq. is calculated and a study of the steady-state kinetics over a whole range of substrate concentrations is reported. By using novel methods of kinetic analysis, a...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1986
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1986.24.6.387